Translational_Unit

Part:BBa_K1500002:Design

Designed by: Kevin Yang   Group: iGEM14_UChicago   (2014-10-17)


IPTG promoter-GFP (IG) backbone


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


Design Notes

The back two cut sites may be cut and mutator genes PCR'd from wild-type may then be inserted into this plasmid.


Source

IPTG promoter was cloned from registry plasmid BBa_J04500, RBS.3 was designed into the primer for cloning IPTG, and these were cloned into reporter GFP, cutting the front two cut sites.

References